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KMID : 0861420120160020131
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Korean Journal of Nuclear Medicine Technology
2012 Volume.16 No. 2 p.131 ~ p.134
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Study on Labeling Efficiency of 99mTc-HMPAO
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Hyeon Jun-Ho
Lim Hyun-Jin
Kim Ha-Kyoon
Cho Seong-Wook
Kim Jin-Eui
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Abstract
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Purpose:The labeling efficiency of radiopharmaceuticals in nuclear medicine is important in terms of accuracy
and reliability of the examination. Usually 99mTc-HMPAO used for brain SPECT scan is chemically unstable
since lots of impurities are existing. Therefore, occurrence of loss of labeling efficiency is easy to appear. In this
paper, labeling and use of 99mTc-HMPAO should be helpful through experiments on factors affecting the labeling
efficiency of 99mTc-HMPAO.
Materials and Methods: Domestic HMPAO vials (Dong-A) used for brain SPECT
scan were tested. Domestic Samyeong Generator 55.5 GBq (1,500 mCi), TLC measurement sets (ITLC-SG,
butanone, saline, TLC chamber) and radio-TLC scanner (Advantest, Bioscan) were used. In the first experiment,
after eluting generator at 1, 8, 16, 24, 28 hours apart, each eluted 99mTc-pertechnetate were labeled with HMPAO
and the labeling efficiency was measured. In the second experiment, after eluting 99mTc-pertechnetate
from a generator, 99mTc-pertechnetate was drawn at 0, 1, 3, 6 hours. And each drawn 99mTc-pertechnetate were
labeled with HMPAO for measuring labeling efficiency. In the third experiment, labeling efficiency was
measured at 0, 0.5, 3, 5, 7 hours after labeling 99mTc-HMPAO.
Results:In the first experiment, measured values
were appeared 95.05, 94.64, 94.94, 95.64, 96.76% in passing order of time. In the second experiment,
measured values were appeared 94.38, 94.23, 93.26, 91.03% in passing order of time. In the third
experiment, measured values were appeared 95.76, 94.17, 88.19, 83.6, 76.86% in passing order of time.
Conclusion:In the first experiment of this paper, labeling efficiency of 99mTc-HMPAO labeled with
99mTc-pertechnetate eluted after 24 hours from first elution. Additional experiments will be needed to discuss for
usability. In the second experiment, the labeling efficiency was slightly decreased in chronological order, but
it was measured higher than 90%. Also, additional experiments will be needed to discuss for usability. In the
third experiment, the labeling efficiency was decreased considerably. Especially, within 3 hours after the
labeling is recommended to use 99mTc-HMPAO.
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KEYWORD
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99mTc-HMPAO , Labeling efficiency , TLC
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